Testosterone Free & Total Testing

Testosterone Free & Total Testing is a laboratory test you can order directly. Testosterone Free & Total Testing measures the amount of testosterone in your blood. Total testosterone includes both bound and free hormone, while free testosterone measures the unbound, active portion. It is often ordered to evaluate symptoms like low sex drive, fatigue, or infertility.

Sample type
blood
Category
Hormone
Turnaround
1-3 business days
Price
$198
Referral
Not required

Testosterone Free & Total Testing measures the amount of testosterone in your blood. Total testosterone includes both bound and free hormone, while free testosterone measures the unbound, active portion. It is often ordered to evaluate symptoms like low sex drive, fatigue, or infertility.

What it measures

  • Total Testosterone — The overall amount of testosterone in your blood, including that attached to proteins.
  • Free Testosterone — The small fraction of testosterone that is not attached to proteins and is available for your body to use.
  • Sex Hormone-Binding Globulin (SHBG) — A protein that binds testosterone; sometimes measured to help calculate free testosterone.
  • Albumin — Another protein that binds testosterone; may be used in some free testosterone calculations.

Who it is for

This test is typically ordered by a doctor for men with symptoms of low testosterone, such as decreased sex drive, erectile dysfunction, or fatigue. It may also be used for women with signs of excess testosterone, like acne or irregular periods.

Preparation

Fasting is not usually required, but testosterone levels are highest in the morning. Your doctor may ask you to have your blood drawn before 10 a.m. for the most accurate results.

Results and what they mean

Results are reported as numbers for total and free testosterone, often with a reference range. Your clinician will interpret these values along with your symptoms and medical history. This information is not medical advice; only a healthcare provider can diagnose or treat any condition.

How to order this test

  1. Choose the test and place a confidential order online.
  2. Select a certified collection site and pick a time that suits you.
  3. Have your specimen collected — most draws take only a few minutes.
  4. Review results in a secure portal, typically within 1–3 days.

Preparation, hours, and pricing vary by site; confirm details directly with the lab before your visit.

Availability by state

Direct-access online ordering for the testosterone free & total testing is available in most states. It is currently unavailable in 6 states:

Everywhere else you can order confidentially and collect your sample at a certified site. Browse by state →

Frequently asked questions

Do I need to fast for this test?

No, fasting is not typically necessary. However, morning testing is recommended because testosterone levels vary throughout the day.

How long does it take to get results?

Results are usually available within 1 to 3 business days.

Can I take this test if I'm on testosterone therapy?

Yes, but tell your doctor. They will interpret results based on your treatment and may adjust timing.

What does 'free' testosterone mean?

Free testosterone is the portion not bound to proteins, which is the form your body can use. It can be low even when total testosterone is normal.

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