ACTH Test
ACTH Test is a laboratory test you can order directly. An ACTH test measures the amount of adrenocorticotropic hormone in your blood. This hormone, made by the pituitary gland, tells your adrenal glands to produce cortisol. The test helps evaluate conditions like adrenal insufficiency, Cushing's syndrome, or pituitary disorders.
- Sample type
- blood
- Category
- Hormone
- Turnaround
- 1-3 business days
- Price
- $49
- Referral
- Not required
An ACTH test measures the amount of adrenocorticotropic hormone in your blood. This hormone, made by the pituitary gland, tells your adrenal glands to produce cortisol. The test helps evaluate conditions like adrenal insufficiency, Cushing's syndrome, or pituitary disorders.
What it measures
- ACTH — The level of adrenocorticotropic hormone in your blood.
- Cortisol (often paired) — A hormone from the adrenal glands that ACTH controls; sometimes checked at the same time.
Who it is for
Doctors order this test when they suspect a hormone imbalance, such as too much or too little cortisol, or to monitor a known pituitary or adrenal condition.
Preparation
Your doctor may ask you to fast overnight or to have the test at a specific time, often in the early morning. Sometimes no special preparation is needed.
Results and what they mean
Results are reported as a number with a reference range. Your clinician will interpret the result along with your symptoms and other tests. This is general information, not medical advice; only your healthcare provider can diagnose or treat any condition.
How to order this test
- Choose the test and place a confidential order online.
- Select a certified collection site and pick a time that suits you.
- Have your specimen collected — most draws take only a few minutes.
- Review results in a secure portal, typically within 1–3 days.
Preparation, hours, and pricing vary by site; confirm details directly with the lab before your visit.
Availability by state
Direct-access online ordering for the acth test is available in most states. It is currently unavailable in 6 states:
Everywhere else you can order confidentially and collect your sample at a certified site. Browse by state →
Frequently asked questions
What does an abnormal ACTH level mean?
It can suggest a problem with your pituitary or adrenal glands, but it must be interpreted with other tests and your symptoms.
Does the test require fasting?
Sometimes. Follow your doctor's instructions, as fasting or timing may affect the result.
How long until I get results?
Typically 1–3 business days, but this can vary by lab.
Is this test painful?
It uses a standard blood draw, which may cause brief discomfort.