Cortisol AM & PM Test
This page explains the cortisol am & pm test, what it measures, and how to arrange it. The Cortisol AM & PM Test measures cortisol levels in your blood at two different times of day. Cortisol is a hormone made by the adrenal glands that helps regulate stress response, blood sugar, and metabolism. Doctors order this test to check for conditions like Cushing's syndrome or Addison's disease, which affect cortisol production.
- Sample type
- blood
- Category
- Hormone
- Turnaround
- 1-3 business days
- Price
- $129
- Referral
- Not required
The Cortisol AM & PM Test measures cortisol levels in your blood at two different times of day. Cortisol is a hormone made by the adrenal glands that helps regulate stress response, blood sugar, and metabolism. Doctors order this test to check for conditions like Cushing's syndrome or Addison's disease, which affect cortisol production.
What it measures
- Cortisol (morning) — The level of cortisol in your blood drawn in the morning, when it is usually highest.
- Cortisol (evening) — The level of cortisol in your blood drawn in the evening, when it is usually lower.
- Diurnal variation — The difference between morning and evening cortisol levels, which helps show if your daily rhythm is normal.
Who it is for
This test is typically ordered by an endocrinologist or primary care doctor when you have symptoms of a hormone imbalance, such as unexplained weight gain, fatigue, or high blood pressure.
Preparation
No special preparation is usually needed, but you may be asked to avoid eating, drinking caffeine, or exercising for a few hours before each blood draw. Your doctor will give you specific timing instructions for the morning and evening samples.
Results and what they mean
Results are reported as two cortisol values with reference ranges for the time of day. Your clinician will interpret these numbers along with your symptoms and other tests. This information is not medical advice; only a healthcare professional can diagnose or treat any condition.
How to order this test
- Choose the test and place a confidential order online.
- Select a certified collection site and pick a time that suits you.
- Have your specimen collected — most draws take only a few minutes.
- Review results in a secure portal, typically within 1–3 days.
Preparation, hours, and pricing vary by site; confirm details directly with the lab before your visit.
Availability by state
Direct-access online ordering for the cortisol am & pm test is available in most states. It is currently unavailable in 6 states:
Everywhere else you can order confidentially and collect your sample at a certified site. Browse by state →
Frequently asked questions
Why do I need two blood draws?
Cortisol levels naturally rise and fall throughout the day. Testing both morning and evening helps show whether your daily pattern is normal.
Does this test require fasting?
Fasting is not always required, but your doctor may ask you to avoid food, caffeine, or exercise before the draws. Follow your specific instructions.
What can affect my cortisol results?
Stress, sleep, medications, pregnancy, and recent illness can all affect cortisol levels. Tell your doctor about any of these factors.
How long until I get my results?
Results are typically available within 1 to 3 business days. Your doctor's office will contact you about the findings.