Cortisol Levels Blood Test
Cortisol Levels Blood Test can be requested without a referral, and this overview covers what to expect. A cortisol levels blood test measures the amount of cortisol, a hormone your adrenal glands make, in your blood. It is ordered to check for conditions such as Addison's disease or Cushing's syndrome, and to evaluate symptoms like fatigue, weight changes, or high blood pressure.
- Sample type
- blood
- Category
- Hormone
- Turnaround
- 1-3 business days
- Price
- $109
- Referral
- Not required
A cortisol levels blood test measures the amount of cortisol, a hormone your adrenal glands make, in your blood. It is ordered to check for conditions such as Addison's disease or Cushing's syndrome, and to evaluate symptoms like fatigue, weight changes, or high blood pressure.
What it measures
- Cortisol — The main hormone being measured; levels change throughout the day.
- Collection time — The time the blood was drawn, because cortisol peaks in the morning and drops later.
- Reference range — The expected range for the time of day your sample was taken.
Who it is for
Doctors order this test when they suspect a cortisol imbalance, such as too much or too little cortisol, based on symptoms like unexplained weight gain or loss, fatigue, or darkening of the skin.
Preparation
Preparation depends on the time your test is scheduled. Often no fasting is required, but your doctor may ask you to avoid certain medications or to have the test at a specific time, such as early morning.
Results and what they mean
Results are reported as a number in micrograms per deciliter (mcg/dL) and are compared to a reference range for the time of day. Your clinician interprets the result along with your symptoms and other tests. This is general information, not medical advice; only your healthcare provider can diagnose or treat any condition.
How to order this test
- Choose the test and place a confidential order online.
- Select a certified collection site and pick a time that suits you.
- Have your specimen collected — most draws take only a few minutes.
- Review results in a secure portal, typically within 1–3 days.
Preparation, hours, and pricing vary by site; confirm details directly with the lab before your visit.
Availability by state
Direct-access online ordering for the cortisol levels blood test is available in most states. It is currently unavailable in 6 states:
Everywhere else you can order confidentially and collect your sample at a certified site. Browse by state →
Frequently asked questions
Do I need to fast for a cortisol blood test?
Usually no fasting is needed, but follow your doctor's instructions because some tests require it.
Why does the time of day matter?
Cortisol levels naturally rise and fall during the day, so the reference range changes depending on when your blood is drawn.
What can affect my cortisol result?
Stress, pregnancy, certain medicines, and the time of day can all affect your cortisol level.
How long do results take?
Results are typically available in 1 to 3 business days.