Nicotine Urine Test
This page explains the nicotine urine test, what it measures, and how to arrange it. A nicotine urine test detects nicotine and its breakdown products in urine. It is ordered to check for tobacco or nicotine use, often for insurance, employment, or smoking-cessation programs.
- Sample type
- urine
- Category
- Drug & Urine Screening
- Turnaround
- 1-3 business days
- Price
- $159
- Referral
- Not required
A nicotine urine test detects nicotine and its breakdown products in urine. It is ordered to check for tobacco or nicotine use, often for insurance, employment, or smoking-cessation programs.
What it measures
- Nicotine — The active substance in tobacco products.
- Cotinine — The main metabolite of nicotine, staying in urine longer.
- 3-Hydroxycotinine — Another nicotine metabolite, sometimes measured.
- Anabasine — A marker that can distinguish tobacco from nicotine replacement.
Who it is for
Healthcare providers, employers, or insurance companies may order this test. It is also used in smoking-cessation programs to monitor abstinence.
Preparation
No fasting or special preparation is needed. A urine sample is collected at any time.
Results and what they mean
Results are reported as positive or negative, or with a numerical level. A positive result means nicotine or its metabolites were detected. Only a qualified clinician can interpret results in context; this is general information, not medical advice.
How to order this test
- Choose the test and place a confidential order online.
- Select a certified collection site and pick a time that suits you.
- Have your specimen collected — most draws take only a few minutes.
- Review results in a secure portal, typically within 1–3 days.
Preparation, hours, and pricing vary by site; confirm details directly with the lab before your visit.
Availability by state
Direct-access online ordering for the nicotine urine test is available in most states. It is currently unavailable in 6 states:
Everywhere else you can order confidentially and collect your sample at a certified site. Browse by state →
Frequently asked questions
How long does nicotine stay in urine?
Nicotine is usually detectable for 1 to 3 days after use. Cotinine can be detected for up to 4 days or longer, depending on use.
Can secondhand smoke cause a positive test?
In rare cases, heavy secondhand smoke exposure may lead to a low positive result, but this is uncommon.
Does nicotine replacement therapy affect the test?
Yes, products like patches or gum can cause a positive nicotine or cotinine result. Anabasine testing can help tell the difference.
Is the test confidential?
Yes, results are handled as private health information and shared only with authorized parties.